A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling.
Ballister ER, Rodgers J, Martial F, Lucas RJ
BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2. PubMed
Ballister ER, Rodgers J, Martial F, Lucas RJ
BMC Biol. 2018 Jan 16;16(1):10. doi: 10.1186/s12915-017-0475-2. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109350 | pEM705 Gs | Wildtype Gαs |
| 109352 | pEM705 Gsi (Ser) | GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant) |
| 109353 | pEM705 Gso (Ser) | GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant) |
| 109354 | pEM705 Gst (Ser) | GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant) |
| 109355 | pEM705 Gsz | GsX chimera with Gαz C-terminus |
| 109356 | pEM705 Gsq | GsX chimera with Gαq C-terminus |
| 109357 | pEM705 Gs12 | GsX chimera with Gα12 C-terminus |
| 109359 | pEM705 Gs13 | GsX chimera with Gα13 C-terminus |
| 109360 | pEM705 Gs15 | GsX chimera with Gα15 C-terminus |
| 109361 | pcDNA3 Rod Opsin | Human rod opsin |
| 109363 | pcDNA3 SWS cone opsin | Human SWS cone opsin with 1D4 tag |
| 109364 | pcDNA3 RL3Am | Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag |
| 109365 | pcDNA3 RL3Sc | Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag |
| 109366 | pcDNA3 RL3m6L2 | Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag |
| 109367 | pcDNA3 RL23Cm6 | Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6 |
| 109368 | pcDNA3 hML23Cm6 | Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6 |
| 109373 | pEM705 Gsi (Cys) | GsX chimera with Gαi C-terminus (Pertussis toxin sensitive) |
| 109375 | pEM705 Gso (Cys) | GsX chimera with Gαo C-terminus (Pertussis toxin sensitive) |
| 109376 | pEM705 Gst (Cys) | GsX chimera with Gαt C-terminus (Pertussis toxin sensitive) |