Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli.
Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. PubMed Article
Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106250 | pLibacceptorV2 | Recombination-mediated cassette exchange with target genomic loci |
| 110241 | E. coli K-12 MG1655_rLP1(atpI-gidB) | E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |
| 110242 | E. coli K-12 MG1655_fLP2(yieN-trkD) | E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |
| 110243 | E. coli K-12 MG1655_fLP3(ybbD-ylbG) | E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |
| 110244 | E. coli K-12 MG1655_fLP4(essQ-cspB) | E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |
| 110245 | E. coli K-12 MG1655_rLP5(nth-ydgR) | E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |
| 110246 | E. coli K-12 MG1655_rLP6(ygcE-ygcF) | E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed. |