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Tunable molecular tension sensors reveal extension-based control of vinculin loading.
LaCroix AS, Lynch AD, Berginski ME, Hoffman BD
Elife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927. PubMed Article

Plasmids from Article

ID Plasmid Purpose
111760tCRMod-GGSGGS5Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain
111761tCRMod-GGSGGS7Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain
111762tCRMod-GGSGGS9Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain
111763VinTS-tCRMod-GGSGGS5Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module
111764VinTS-tCRMod-GGSGGS7Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module
111765VinTS-tCRMod-GGSGGS9Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Antibodies from Article