Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions.
Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J
Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
111704 | Mode #1 Library | Encodes GST-fused human phosphopeptides |
111705 | Mode #2 Library (14-3-3β) | Encodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-P |
111706 | Mode #2 Library (14-3-3σ) | Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P |
111707 | Mode #2 Library (NEDD4 WW2 domain) | Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P |
111708 | Mode #2 Library (NEDD4-2 WW2 domain) | Encodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P |
111880 | pNAS1b split mCherry 14-3-3β | Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain) |
111881 | pNAS1b split mCherry 14-3-3σ | Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain) |
111882 | pNAS1b split mCherry NEDD4 WW2 | Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain) |
111883 | pNAS1b split mCherry NEDD4-2 WW2 | Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain) |
112029 | pNAS1b split mCherry NEDD4 WW2 pos ctrl | Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide) |
112030 | pNAS1b split mCherry NEDD4 WW2 neg ctrl | Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide) |
112031 | pNAS1b split mCherry 14-3-3β ctrl | Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon) |