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Robust CRISPR/Cas9-Mediated Tissue Specific Mutagenesis Reveals Gene Redundancy and Perdurance in Drosophila.
Poe AR, Wang B, Sapar ML, Ji H, Li K, Onabajo T, Fazliyeva R, Gibbs M, Qiu Y, Hu Y, Han C
Genetics. 2018 Nov 30. pii: genetics.118.301736. doi: 10.1534/genetics.118.301736.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112808pAC-U61-SapIgRNA cloning vector for expressing 1-3 gRNAs in Drosophila
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112809pTGC-U6.2PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment
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112810pTGC-U6.3PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment
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112811pAC-U63-tgRNA-RevgRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila
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112812pMGCPCR template vector for amplifying gRNA(F+E)-OstRNA fragment
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112813pDEST-HemmarR2gateway cloning destination vector for expressing enhancer driven CD4-tdTomato
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112814pDEST-HemmarG2gateway cloning destination vector for expressing enhancer driven CD4-tdGFP
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112815pDEST-HemmarI2gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1
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121657pDEST-APIC-Cas9gateway cloning destination vector for expressing enhancer driven Cas9
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Recombinant Antibodies from Article