A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9.
Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D
Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5. PubMed
Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D
Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114005 | psgRNA | express sgRNA. ColE1, Kan |
| 114006 | psgRNAc | express sgRNA, p15A, CRM |
| 115924 | LC-E18 | Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome |
| 115925 | LC-E75 | Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome |
| 115926 | AV04 | Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome |
| 115927 | psgRNA Library | Genome-wide knockdown of E. coli genes |
| 116947 | pOSIP-KL-mcherry | Integrating mCherry into lambda attB in E. coli chromosome. |