Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020. PubMed Article
Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113500 | pGEX-6P-1 p37 | Bacterial expression of GST-tagged p37 |
| 113501 | pGEX-6P-1 p37deltaSEP | Bacterial expression of GST-tagged p37 lacking the SEP domain |
| 113502 | pGEX-6P-1 p37delta2-81 | Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81 |
| 113503 | pGEX-6P-1 p37 SHPmut | Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box |
| 113504 | pGEX-6P-1 p37 deltaUBX | Bacterial expression of GST-tagged p37 lacking the UBX domain |
| 113505 | pGEX-4T-1 UBXN2A | Bacterial expression of GST-tagged UBXN2A |
| 113506 | pcDNA5FRT/TO PP1gamma-2*Strep | Expresses dual Strep-tagged PP1gamma in mammalian cells |
| 113510 | pFL His-p97 | Expression of His-p97 in insect cells (Baculovirus system) |
| 113511 | pFL His-SDS22 / PP1gamma | Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system) |
| 113512 | pFL His-SDS22 | Expression of His-SDS22 in insect cells (Baculovirus system) |
| 113513 | pFL His-I3 | Expression of His-I3 in insect cells (Baculovirus system) |
| 113514 | pFL I3 | Expression of I3 in insect cells (Baculovirus system) |
| 113515 | pFL His-I3 V41A / W43A | Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system) |
| 113516 | pFL His-mEos3.2-I3 | Expression of His-Eos-I3 in insect cells (Baculovirus system) |
| 117107 | pET41b+_Ufd1-His | Production of recombinant Ufd1-His in E.coli |
| 117108 | pET41b+_Npl4 | Production of recombinant Npl4 in E.coli |
| 169020 | pET15b His-p97 | Expresses His-p97 in bacteria |
| 169021 | pET15b p97-Strep-His E314Amb | Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria |
| 169022 | pET15b p97-His D592Amb | Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria |