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Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O
Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117919pX330-SpCas9-NGExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.
119861pSI-Target-AID-NGExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.