A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. PubMed Article
Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119129 | eGFP L202 Reporter | Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter. |
| 119130 | eGFP L138 Reporter | Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter. |
| 119132 | eGFP L202 gRNA | gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP. |
| 119133 | eGFP L138 gRNA | gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP. |
| 119135 | A3Bi-Cas9n-UGI | Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction |
| 119136 | A3C-Cas9n-UGI | Base editor made from APOBEC3C |
| 119137 | A3D-Cas9n-UGI | Base editor made from APOBEC3D |
| 119138 | A3F-Cas9n-UGI | Base editor made from APOBEC3F |
| 119139 | A3G-Cas9n-UGI | Base editor made from APOBEC3G |
| 119140 | A3H HapI-Cas9n-UGI | Base editor made from APOBEC3H Haplotype I |
| 119141 | A3H HapII-Cas9n-UGI | Base editor made from APOBEC3H Haplotype II |
| 119142 | A3H HapII R175/6E-Cas9n-UGI | Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE |