CRISPR-Cas12a-assisted PCR tagging of mammalian genes.
Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M
J Cell Biol. 2020 Jun 1;219(6). pii: 151766. doi: 10.1083/jcb.201910210. PubMed Article
Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M
J Cell Biol. 2020 Jun 1;219(6). pii: 151766. doi: 10.1083/jcb.201910210. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119980 | pMaCTag-01 | Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP |
| 119981 | pMaCTag-02 | Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP |
| 119982 | pMaCTag-03 | Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3 |
| 119983 | pMaCTag-04 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1 |
| 119984 | pMaCTag-05 | Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP |
| 119985 | pMaCTag-06 | Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP |
| 119986 | pMaCTag-33 | Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG |
| 119987 | pMaCTag-08 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3 |
| 119988 | pMaCTag-09 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus |
| 119989 | pMaCTag-10 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk |
| 119990 | pMaCTag-11 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i |
| 119991 | pMaCTag-12 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet |
| 119992 | pMaCTag-13 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry |
| 119993 | pMaCTag-14 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5 |
| 119994 | pMaCTag-15 | Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670 |
| 119995 | pMaCTag-16 | Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3 |
| 119996 | pMaCTag-17 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b |
| 119997 | pMaCTag-18 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5 |
| 119998 | pMaCTag-34 | Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG |
| 119999 | pMaCTag-20 | Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag |
| 120000 | pMaCTag-21 | Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag |
| 120001 | pMaCTag-22 | Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP |
| 120002 | pMaCTag-23 | Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo |
| 120003 | pMaCTag-24 | Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP |
| 120004 | pMaCTag-25 | Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID |
| 120005 | pMaCTag-26 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover |
| 120006 | pMaCTag-27 | Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA |
| 120007 | pMaCTag-28 | Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA |
| 120008 | pMaCTag-29 | Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II |
| 120009 | pMaCTag-30 | Template for C-terminal PCR tagging of mammalian genes (without selection) with VSV-G |
| 120010 | pMaCTag-31 | Template for C-terminal PCR tagging of mammalian genes (without selection) with V5 |
| 120011 | pMaCTag-32 | Template for C-terminal PCR tagging of mammalian genes (without selection) with S-Tag |
| 120012 | pMaCTag-P01 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP |
| 120013 | pMaCTag-P02 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP |
| 120014 | pMaCTag-P03 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3 |
| 120015 | pMaCTag-P04 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1 |
| 120016 | pMaCTag-P05 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP |
| 120017 | pMaCTag-P06 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP |
| 120018 | pMaCTag-P33 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG |
| 120019 | pMaCTag-P08 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3 |
| 120020 | pMaCTag-P09 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus |
| 120021 | pMaCTag-P10 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk |
| 120022 | pMaCTag-P11 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i |
| 120023 | pMaCTag-P12 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet |
| 120024 | pMaCTag-P13 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry |
| 120025 | pMaCTag-P14 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5 |
| 120026 | pMaCTag-P15 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670 |
| 120027 | pMaCTag-P16 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3 |
| 120028 | pMaCTag-P17 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b |
| 120029 | pMaCTag-P18 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5 |
| 120030 | pMaCTag-P34 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG |
| 120031 | pMaCTag-P20 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag |
| 120032 | pMaCTag-P21 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag |
| 120033 | pMaCTag-P22 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP |
| 120034 | pMaCTag-P23 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo |
| 120035 | pMaCTag-P24 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP |
| 120036 | pMaCTag-P25 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID |
| 120037 | pMaCTag-P26 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover |
| 120038 | pMaCTag-P27 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA |
| 120039 | pMaCTag-P28 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA |
| 120040 | pMaCTag-P29 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II |
| 120041 | pMaCTag-P30 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G |
| 120042 | pMaCTag-P31 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5 |
| 120043 | pMaCTag-P32 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag |
| 120044 | pMaCTag-Z01 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP |
| 120045 | pMaCTag-Z02 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP |
| 120046 | pMaCTag-Z03 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3 |
| 120047 | pMaCTag-Z04 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1 |
| 120048 | pMaCTag-Z05 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP |
| 120049 | pMaCTag-Z06 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP |
| 120050 | pMaCTag-Z33 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG |
| 120051 | pMaCTag-Z08 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3 |
| 120052 | pMaCTag-Z09 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus |
| 120053 | pMaCTag-Z10 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk |
| 120054 | pMaCTag-Z11 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i |
| 120055 | pMaCTag-Z12 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet |
| 120056 | pMaCTag-Z13 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry |
| 120057 | pMaCTag-Z14 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5 |
| 120058 | pMaCTag-Z15 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670 |
| 120059 | pMaCTag-Z16 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3 |
| 120060 | pMaCTag-Z17 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b |
| 120061 | pMaCTag-Z18 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5 |
| 120062 | pMaCTag-Z34 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG |
| 120063 | pMaCTag-Z20 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag |
| 120064 | pMaCTag-Z21 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag |
| 120065 | pMaCTag-Z22 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP |
| 120066 | pMaCTag-Z23 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo |
| 120067 | pMaCTag-Z24 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP |
| 120068 | pMaCTag-Z25 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID |
| 120069 | pMaCTag-Z26 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover |
| 120070 | pMaCTag-Z27 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA |
| 120071 | pMaCTag-Z28 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA |
| 120072 | pMaCTag-Z29 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II |
| 120073 | pMaCTag-Z30 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G |
| 120074 | pMaCTag-Z31 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5 |
| 120075 | pMaCTag-Z32 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag |
| 124786 | pMaCTag-07 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen |
| 124787 | pMaCTag-19 | Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen |
| 124788 | pMaCTag-P07 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen |
| 124789 | pMaCTag-P19 | Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen |
| 124790 | pMaCTag-Z07 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen |
| 124791 | pMaCTag-Z19 | Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen |