The interaction between ORF18 and ORF30 is required for late gene expression in Kaposi's sarcoma-associated herpesvirus.
Castaneda AF, Glaunsinger BA
J Virol. 2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18. PubMed Article
Castaneda AF, Glaunsinger BA
J Virol. 2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120372 | ORF18-3xFLAG pCDNA4 | Expresses KSHV ORF18 with Cterm 3xFLAG tag in mammalian cells |
| 120374 | UL79-3xFLAG pCDNA4 | Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells |
| 120375 | UL91-2xStrep pCDNA4 | Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells |
| 120376 | 2xStrep-ORF34 pCDNA4 | Expresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cells |
| 120377 | K8.1 Pr pGL4.16 | Firefly luciferase reporter with KSHV K8.1 late gene promoter |
| 120378 | ORF57 Pr pGL4.16 | Firefly luciferase reporter with KSHV ORF57 early gene promoter |
| 129742 | ORF24-2xStrep pCDNA4 | Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells |
| 129743 | ORF30-2xStrep pCDNA4 | Expresses KSHV ORF30 with Cterm 2xStrep tag in mammalian cells |
| 129744 | ORF31-2xStrep pCDNA4 | Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells |
| 129746 | pLVX-TetOneZeo-ORF18(WT)-3xFLAG | Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells |
| 129758 | pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG | Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells |
| 129759 | UL79(E48A_L49A)-3xFLAG pCDNA4 | Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells |
| 129777 | ORF18(L29A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cells |
| 129778 | ORF18(E36A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (E36A) with Cterm 3xFLAG tag in mammalian cells |
| 129779 | ORF18(L37A)-3xFLAG pCDNA4 | Expresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cells |
| 129780 | ORF18(N63A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (N63A) with Cterm 3xFLAG tag in mammalian cells |
| 129781 | ORF18(G65A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cells |
| 129782 | ORF18(L72A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L72A) with Cterm 3xFLAG tag in mammalian cells |
| 129783 | ORF18(R74A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (R74A) with Cterm 3xFLAG tag in mammalian cells |
| 129784 | ORF18(K75A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cells |
| 129785 | ORF18(W81A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (W81A) with Cterm 3xFLAG tag in mammalian cells |
| 129786 | ORF18 (Y85A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (Y85A) with Cterm 3xFLAG tag in mammalian cells |
| 129787 | ORF18(L107A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cells |
| 129795 | ORF18(G130A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G130A) with Cterm 3xFLAG tag in mammalian cells |
| 129796 | ORF18(G145A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G145A) with Cterm 3xFLAG tag in mammalian cells |
| 129798 | ORF18(N146A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells |
| 129799 | ORF18(L151A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L151A) with Cterm 3xFLAG tag in mammalian cells |
| 129800 | ORF18(G152A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G152A) with Cterm 3xFLAG tag in mammalian cells |
| 129801 | ORF18(R158A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cells |
| 129802 | ORF18(L159A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L159A) with Cterm 3xFLAG tag in mammalian cells |
| 129803 | ORF18(W170)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (W170A) with Cterm 3xFLAG tag in mammalian cells |
| 129804 | ORF18(G171A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G171A) with Cterm 3xFLAG tag in mammalian cells |
| 129805 | ORF18(E176A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (E176A) with Cterm 3xFLAG tag in mammalian cells |
| 129806 | ORF18(R180A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (R180A) with Cterm 3xFLAG tag in mammalian cells |
| 129807 | ORF18(L191A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L191A) with Cterm 3xFLAG tag in mammalian cells |
| 129808 | ORF18(G214A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G214A) with Cterm 3xFLAG tag in mammalian cells |
| 129809 | ORF18(G228A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells |
| 129810 | ORF18(E36A_L37A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (E36A_L37A) with Cterm 3xFLAG tag in mammalian cells |
| 129811 | ORF18(R74A_K75A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells |
| 129812 | ORF18(G145A_N146A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cells |
| 129813 | ORF18(L151A_G152A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (L151A_G152A) with Cterm 3xFLAG tag in mammalian cells |
| 129814 | ORF18(R158A_L159A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells |
| 129815 | ORF18(W170A_G171A)-3xFLAG pCDNA4 | Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells |