Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus.
Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G
EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. PubMed Article
Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, Pelechano V, Cittaro D, Tonon G
EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 10.15252/embj.201899179. Epub 2018 Oct 29. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123292 | pEF-DEST51 LacZ | Mammalian expression of control truncated LacZ |
| 123293 | pEF-DEST51 PNRC1 | Expression of PNRC1 in mammalian cells |
| 123294 | pEF-DEST51 HA-PNRC1 | Mammalian expression of PNRC1 N-HA |
| 123295 | pEF-DEST51 GFP-PNRC1 | Mammalian expression of PNRC1 N-GFP |
| 123297 | pLenti4 Blast RFP-PNRC1 | Mammalian expression of PNRC1 N-RFP |
| 123298 | pLenti4 Blast RFP-PNRC1 W300A | Mammalian expression of PNRC1-W300A mutant N-RFP |
| 123299 | pLenti c-MYC-DDK-Puro GFP | Mammalian expression of GFP C-MYC-FLAG |
| 123300 | pLenti C-MYC-DDK Puro PNRC1 | Mammalian expression of PNRC1 C-MYC-FLAG |
| 123301 | pLenti C-MYC-DDK Puro PNRC1-W300A | Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG |
| 123306 | pLKO.1 Blast NT shRNA | Expression of a non-targeting shRNA |