Repurposing CRISPR-Cas12b for mammalian genome engineering.
Teng F, Cui T, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W
Cell Discov. 2018 Nov 27;4:63. doi: 10.1038/s41421-018-0069-3. eCollection 2018. PubMed Article
Teng F, Cui T, Feng G, Guo L, Xu K, Gao Q, Li T, Li J, Zhou Q, Li W
Cell Discov. 2018 Nov 27;4:63. doi: 10.1038/s41421-018-0069-3. eCollection 2018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121945 | pCAG-AaCas12b-2AeGFP | Mammalian expression, Genome editing |
| 121946 | pCAG-AkCas12b-2AeGFP | Mammalian expression, Genome editing |
| 121947 | pCAG-AmCas12b-2AeGFP | Mammalian expression, Genome editing |
| 121948 | pCAG-BsCas12b-2AeGFP | Mammalian expression, Genome editing |
| 121949 | BPK2014-AaCas12b | E. coli expression, protein purification |
| 121950 | BPK2013-AkCas12b | E. coli expression, protein purification |
| 121951 | BPK2014-AmCas12b | E. coli expression, protein purification |
| 121952 | BPK2013-BsCas12b | E. coli expression, protein purification |
| 121953 | pUC19-U6-AagRNA | Mammalian expression, Genome editing, gRNA scaffold |
| 121954 | pUC19-U6-AasgRNA | Mammalian expression, Genome editing, gRNA scaffold |
| 121955 | pUC19-U6-AksgRNA | Mammalian expression, Genome editing, gRNA scaffold |
| 121956 | pUC19-U6-AmsgRNA | Mammalian expression, Genome editing, gRNA scaffold |
| 121957 | pUC19-U6-BssgRNA | Mammalian expression, Genome editing, gRNA scaffold |
| 121958 | pUC19-U6-AasgRNA3.8 | Mammalian expression, Genome editing, gRNA scaffold |
| 121959 | pUC19-U6-AasgRNA3.8.3 | Mammalian expression, Transcription regulation, gRNA scaffold |
| 121961 | pCAG-AaCas12b(R911A)-2AeGFP | Mammalian expression, Transcription regulation, deactivated AaCas12b |
| 121962 | pCAG-AaCas12b(D977A)-2AeGFP | Mammalian expression, Transcription regulation, deactivated AaCas12b |
| 121963 | pCAG-MCP-VP64-2AmScarlet | Mammalian expression, Transcription regulation module |
| 121964 | pCAG-MCP-VPR-2AmScarlet | Mammalian expression, Transcription regulation module |