Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H, Nikaido I
Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi: 10.1126/sciadv.aaz6699. eCollection 2020 Jun. PubMed Article
Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, Nakano K, Saitoh N, Liu Z, Yamamoto T, Okamura T, Ohkawa Y, Kimura H, Nikaido I
Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi: 10.1126/sciadv.aaz6699. eCollection 2020 Jun. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122302 | pCas9n-Nanog-L | Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells |
| 122303 | pCas9n-Nanog-R | Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells |
| 122304 | px335-EF-Slc2a3-L | Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells |
| 122305 | px335-EF-Slc2a3-R | Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells |
| 122306 | px335-sgRNA-EF(Cas9wt)-Rif1 | Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells |
| 122307 | px335-sgRNA-EF(Cas9n)-mHdac1-L2 | Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells |
| 122308 | px335-sgRNA-EF(Cas9n)-mHdac1-R2 | Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells |
| 122309 | px335-sgRNA-EF(Cas9n)-mHes1-L | Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells |
| 122310 | px335-sgRNA-EF(Cas9n)-mHes1-R | Expresses sgRNA targeting mouse Hes1 and Cas9 nickase in mammalian cells |
| 122311 | px335-sgRNA-EF(Cas9n)-Peg3-L | Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells |
| 122312 | px335-sgRNA-EF(Cas9n)-Peg3-R | Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells |
| 122313 | px335-sgRNA-EF(Cas9n)-Rad21-L | Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells |
| 122314 | px335-sgRNA-EF(Cas9n)-Rad21-R | Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells |
| 122315 | px335-sgRNA-EF(Cas9n)-Ssrp1-L | Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells |
| 122316 | px335-sgRNA-EF(Cas9n)-Ssrp1-R | Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells |
| 122317 | px335-sgRNA-EF(Cas9n)-Trim27-L | Expresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cells |
| 122318 | px335-sgRNA-EF(Cas9n)-Trim27-R | Expresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cells |
| 122319 | px335-sgRNA-EF(Cas9n)-Ctcf-L | Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells |
| 122320 | px335-sgRNA-EF(Cas9n)-Ctcf-R | Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells |
| 122321 | px335-sgRNA-EF(Cas9n)-Dnmt3l-L | Expresses sgRNA targeting mouse Dnmt3l and Cas9 nickase in mammalian cells |
| 122322 | px335-sgRNA-EF(Cas9n)-Dnmt3l-R | Expresses sgRNA targeting mouse Dnmt3l and Cas9 nickase in mammalian cells |
| 122323 | px335-sgRNA-EF(Cas9n)-Etv5-L | Expresses sgRNA targeting mouse Etv5 and Cas9 nickase in mammalian cells |
| 122324 | px335-sgRNA-EF(Cas9n)-Etv5-R | Expresses sgRNA targeting mouse Etv5 and Cas9 nickase in mammalian cells |
| 122325 | px335-sgRNA-EF(Cas9n)-Jade1-L | Expresses sgRNA targeting mouse Jade1 and Cas9 nickase in mammalian cells |
| 122326 | px335-sgRNA-EF(Cas9n)-Jade1-R | Expresses sgRNA targeting mouse Jade1 and Cas9 nickase in mammalian cells |
| 122327 | px335-sgRNA-EF(Cas9n)-Kdm1a-L | Expresses sgRNA targeting mouse Kdm1a and Cas9 nickase in mammalian cells |
| 122328 | px335-sgRNA-EF(Cas9n)-Kdm1a-R | Expresses sgRNA targeting mouse Kdm1a and Cas9 nickase in mammalian cells |
| 122329 | px335-sgRNA-EF(Cas9n)-Ncapd3-L | Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells |
| 122330 | px335-sgRNA-EF(Cas9n)-Ncapd3-R | Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells |
| 122331 | px335-sgRNA-EF(Cas9n)-Npm1-R | Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells |
| 122332 | px335-sgRNA-EF(Cas9n)-Npm1-L | Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells |
| 122333 | px335-sgRNA-EF(Cas9n)-Dnmt3b-R | Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells |
| 122334 | px335-sgRNA-EF(Cas9n)-Dnmt3b-L | Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells |
| 122335 | px335-sgRNA-EF(Cas9n)-Dhx15-R | Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells |
| 122336 | px335-sgRNA-EF(Cas9n)-Dhx15-L | Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells |
| 122337 | px335-sgRNA-EF(Cas9n)-Birc6-L | Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells |
| 122338 | px335-sgRNA-EF(Cas9n)-Birc6-R | Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells |
| 122339 | px335-sgRNA-EF(Cas9n)-Trim28-R | Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells |
| 122340 | px335-sgRNA-EF(Cas9n)-Trim28-L | Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells |
| 122341 | eSpCas9-EF-Gsk3b(new) | Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells |
| 122342 | eSpCas9(1.1)-Cdt1 | Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells |
| 122343 | px335-sgRNA-EF(Cas9n)-Ddx42-L | Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells |
| 122344 | px335-sgRNA-EF(Cas9n)-Ddx42-R | Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells |
| 122345 | px335-sgRNA-EF(Cas9n)-Eif4a1-R | Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells |
| 122346 | px335-sgRNA-EF(Cas9n)-Eif4a1-L | Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells |
| 122347 | pTV-mNanog-tdiRFP670-PEST | Targeting vector for mouse Nanog to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122348 | pTV-mSlc2a3-tdiRFP670-PEST | Targeting vector for mouse Slc2a3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122349 | pTV-mRif1-tdiRFP670-PEST | Targeting vector for mouse Rif1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122350 | pTV-Klf4-tdiRFP670-PEST | Targeting vector for mouse Klf4 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122351 | pTV-mHdac1-tdiRFP670-PEST2 | Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122352 | pTV-mHes1-tdiRFP670-PEST | Targeting vector for mouse Hes1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122353 | pTV-Peg3-tdiRFP670-PEST | Targeting vector for mouse Peg3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122354 | pTV-Rad21-tdiRFP670-PEST | Targeting vector for mouse Rad21 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122355 | pTV-Ssrp1-tdiRFP670-PEST | Targeting vector for mouse Ssrp1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122356 | pTV-Trim27-tdiRFP670-PEST | Targeting vector for mouse Trim27 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122357 | pTV-Ctcf-tdiRFP670-PEST | Targeting vector for mouse Ctcf to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122358 | pTV-Dnmt3l-tdiRFP670-PEST | Targeting vector for mouse Dnmt3l to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122359 | pTV-Etv5-tdiRFP670-PEST | Targeting vector for mouse Etv5 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122360 | pTV-Jade1-tdiRFP670-PEST | Targeting vector for mouse Jade1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122361 | pTV-Kdm1a-tdiRFP670-PEST | Targeting vector for mouse Kdm1a to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122362 | pTV-Ncapd3-tdiRFP670-PEST | Targeting vector for mouse Ncapd3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122363 | pTV-Npm1-tdiRFP670-PEST | Targeting vector for mouse Npm1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122364 | pTV-Dnmt3b-tdiRFP670-PEST | Targeting vector for mouse Dnmt3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122365 | pTV-Dhx15-tdiRFP670-PEST | Targeting vector for mouse Dhx15 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122366 | pTV-Birc6-tdiRFP670-PEST | Targeting vector for mouse Birc6 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122367 | pTV-Trim28-tdiRFP670-PEST | Targeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122368 | pTV-Gsk3b-tdiRFP670-PEST | Targeting vector for mouse Gsk3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122369 | pTV-Cdt1-tdiRFP670-PEST | Targeting vector for mouse Cdt1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122370 | pTV-Ddx42-tdiRFP670-PEST | Targeting vector for mouse Ddx42 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon. |
| 122371 | pTV-Met-Eif4a1-tdiRFP670-PEST | Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon. |
| 122372 | pKLV-PGKpuro2ABFP | Expresses puro-2A-BFP in mammalian cells |
| 122373 | pCAG-mTagBFP2 | Expresses mTagBFP2 in mammalian cells |
| 122374 | pLR5-CBh-dCas9-hUtx-IRES-Hyg | Expresses dCas9-hUtx-IRES-Hyg in mammalian cells |
| 122375 | pLR5-CBh-dCas9-hEzh2-IRES-Hyg | Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells |