CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans.
Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, Silverman GA, Luke CJ
PLoS One. 2019 Mar 26;14(3):e0214257. doi: 10.1371/journal.pone.0214257. eCollection 2019. PubMed Article
Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, Silverman GA, Luke CJ
PLoS One. 2019 Mar 26;14(3):e0214257. doi: 10.1371/journal.pone.0214257. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122533 | pJR2956 | Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2 |
| 122534 | pLS2965 | Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2 |
| 122535 | pBT2999 | Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2 |
| 122536 | pBT3000 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2 |
| 122537 | pBT3003 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2 |
| 122538 | pBT3007 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2 |
| 122539 | pBT3011 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2 |
| 122540 | pBT3014 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2 |
| 122541 | pBT3019 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2 |
| 122542 | pBT3024 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2 |
| 122543 | pBT3035 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2 |
| 122544 | pBT3037 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2 |
| 122545 | pBT3038 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2 |
| 122546 | pBT3043 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2 |
| 122547 | pBT3047 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2 |
| 122548 | pBT3102 | Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2 |
| 122549 | pJR2953 | Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2 |
| 122550 | pJR2955 | Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2 |