Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.
Agrotis A, Pengo N, Burden JJ, Ketteler R
Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. PubMed Article
Agrotis A, Pengo N, Burden JJ, Ketteler R
Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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122842 | pDEST-CMV-N-EGFP | N-terminal EGFP tag. Gateway destination vector for mammalian expression. |
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122843 | pDEST-CMV-polysite | Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette. |
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122844 | pDEST-CMV-C-EGFP | C-terminal EGFP tag. Gateway destination vector for mammalian expression. |
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122845 | pDEST-CMV-3xFLAG-gateway-EGFP | N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression. |
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122848 | pLVpuro-CMV-N-EGFP | N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells. |
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123069 | pDONR223 LC3A WT | Gateway entry clone encoding human MAP1LC3A wild-type |
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123070 | pDONR223 LC3A G120A | Gateway entry clone encoding human MAP1LC3A G120A |
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123071 | pDONR223 LC3A G120 | Gateway entry clone encoding human MAP1LC3A G120 |
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123072 | pDONR223 LC3B WT | Gateway entry clone encoding human MAP1LC3B wild-type |
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123073 | pDONR223 LC3B G120A | Gateway entry clone encoding human MAP1LC3B G120A |
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123074 | pDONR223 LC3B G120 | Gateway entry clone encoding human MAP1LC3B G120 |
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123075 | pDONR223 LC3B2 WT | Gateway entry clone encoding human MAP1LC3B2 wild-type |
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123076 | pDONR223 LC3B2 G120A | Gateway entry clone encoding human MAP1LC3B2 G120A |
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123077 | pDONR223 LC3B2 G120 | Gateway entry clone encoding human MAP1LC3B2 G120 |
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123078 | pDONR223 LC3C WT | Gateway entry clone encoding human MAP1LC3C wild-type |
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123079 | pDONR223 LC3C G126 | Gateway entry clone encoding human MAP1LC3C G126 |
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123080 | pDONR223 GABARAP WT | Gateway entry clone encoding human GABARAP wild-type |
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123081 | pDONR223 GABARAP G116A | Gateway entry clone encoding human GABARAP G116A |
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123082 | pDONR223 GABARAP G116 | Gateway entry clone encoding human GABARAP G116 |
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123083 | pDONR223 GABARAPL1 WT | Gateway entry clone encoding human GABARAPL1 wild-type |
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123084 | pDONR223 GABARAPL1 G116A | Gateway entry clone encoding human GABARAPL1 G116A |
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123085 | pDONR223 GABARAPL1 G116 | Gateway entry clone encoding human GABARAPL1 G116 |
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123086 | pDONR223 GABARAPL2 WT | Gateway entry clone encoding human GABARAPL2 wild-type |
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123087 | pDONR223 GABARAPL2 G116A | Gateway entry clone encoding human GABARAPL2 G116A |
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123088 | pDONR223 GABARAPL2 G116 | Gateway entry clone encoding human GABARAPL2 G116 |
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123089 | pCMV 3xFLAG-LC3A WT | Expresses 3xFLAG-LC3A wild-type in mammalian cells. |
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123090 | pCMV 3xFLAG-LC3A G120A | Expresses 3xFLAG-LC3A G120A in mammalian cells. |
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123091 | pCMV 3xFLAG-LC3A G120 | Expresses 3xFLAG-LC3A G120 in mammalian cells. |
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123092 | pCMV 3xFLAG-LC3B WT | Expresses 3xFLAG-LC3B wild-type in mammalian cells. |
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123093 | pCMV 3xFLAG-LC3B G120A | Expresses 3xFLAG-LC3B G120A in mammalian cells. |
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123094 | pCMV 3xFLAG-LC3B G120 | Expresses 3xFLAG-LC3B G120 in mammalian cells. |
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123095 | pCMV 3xFLAG-LC3C WT | Expresses 3xFLAG-LC3C wild-type in mammalian cells. |
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123096 | pCMV 3xFLAG-LC3C G126 | Expresses 3xFLAG-LC3C G126 in mammalian cells. |
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123097 | pCMV 3xFLAG-GABARAP WT | Expresses 3xFLAG-GABARAP wild-type in mammalian cells. |
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123098 | pCMV 3xFLAG-GABARAP G116A | Expresses 3xFLAG-GABARAP G116A in mammalian cells. |
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123099 | pCMV 3xFLAG-GABARAP G116 | Expresses 3xFLAG-GABARAP G116 in mammalian cells. |
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123100 | pCMV 3xFLAG-GABARAPL1 WT | Expresses 3xFLAG-GABARAPL1 wild-type in mammalian cells. |
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123101 | pCMV 3xFLAG-GABARAPL1 G116A | Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells. |
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123102 | pCMV 3xFLAG-GABARAPL1 G116 | Expresses 3xFLAG-GABARAPL1 G116 in mammalian cells. |
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123103 | pCMV 3xFLAG-GABARAPL2 WT | Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells. |
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123104 | pCMV 3xFLAG-GABARAPL2 G116A | Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells. |
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123105 | pCMV 3xFLAG-GABARAPL2 G116 | Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells. |
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123106 | pDEST-CMV 3xFLAG-LC3A-GFP | Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A |
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123107 | pDEST-CMV 3xFLAG-LC3B-GFP | Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B |
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123108 | pDEST-CMV 3xFLAG-LC3B-G120A-GFP | Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity. |
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123109 | pDEST-CMV 3xFLAG-LC3B2-GFP | Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2 |
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123110 | pDEST-CMV 3xFLAG-LC3C-GFP | Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C |
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123111 | pDEST-CMV 3xFLAG-GABARAP-GFP | Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP |
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123112 | pDEST-CMV 3xFLAG-GABARAPL1-GFP | Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1 |
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123113 | pDEST-CMV 3xFLAG-GABARAPL2-GFP | Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2 |
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123114 | pDEST-CMV GFP-LC3B WT | Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes. |
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123115 | pDEST-CMV GFP-LC3B G120A | Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes. |
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123116 | pDEST-CMV GFP-LC3B G120 | Expresses EGFP-LC3B G120 in mammalian cells. |
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123117 | pDEST-CMV GFP-GABARAPL1 WT | Expresses EGFP-GABARAPL1 wild-type in mammalian cells. |
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123118 | pDEST-CMV GFP-GABARAPL1 G116A | Expresses EGFP-GABARAPL1 G116A in mammalian cells. |
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123119 | pDEST-CMV GFP-GABARAPL1 G116 | Expresses EGFP-GABARAPL1 G116 in mammalian cells. |
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123204 | pDONR223 LC3A no-stop | Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging |
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123205 | pDONR223 LC3B no-stop | Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging |
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123206 | pDONR223 LC3B G120A no-stop | Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging |
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123207 | pDONR223 LC3B2 no-stop | Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging |
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123208 | pDONR223 LC3C no-stop | Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging |
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123209 | pDONR223 GABARAP no-stop | Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging |
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123210 | pDONR223 GABARAPL1 no-stop | Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging |
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123211 | pDONR223 GABARAPL2 no-stop | Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging |
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123215 | pDEST-CMV-N-mCherry | N-terminal mCherry tag. Gateway destination vector for mammalian expression. |
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123216 | pDEST-CMV-N-Tandem-mCherry-EGFP | N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression. |
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123221 | pLVpuro-CMV-N-mCherry | N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells. |
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123223 | pLVpuro-CMV-N-3xFLAG | N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells. |
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123230 | pDEST-CMV mCherry-GFP-LC3B WT | Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter. |
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123235 | pDEST-CMV mCherry-GFP-LC3B G120A | Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter. |
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123241 | pLenti PGK Puro GFP-LC3B G120 | Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells. |
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123242 | pLenti PGK Puro GFP-LC3B G120A | Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells. |
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123243 | pLenti PGK Puro GFP-GABARAPL1 G116 | Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells. |
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123245 | pLenti PGK Puro GFP-GABARAPL1 G116A | Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells. |
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