Addgene: Luminescent kinase activity biosensors based on a versatile bimolecular switch.

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Jin Zhang
Herbst et al

Luminescent kinase activity biosensors based on a versatile bimolecular switch.
Herbst KJ, Allen MD, Zhang J
J Am Chem Soc. 2011 Apr 20;133(15):5676-9. doi: 10.1021/ja1117396. Epub 2011 Mar 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
138202	pDNA3-Cerulean-FHA1-NES	Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.
138203	pcDNA3-PKAsub-YPet-NES	Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
138205	pcDNA3-PKAsub(T/A)-YPet-NES	Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
138206	pcDNA3-PKAsub-YPet-Kras	Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
138207	pcDNA3-PKCsub-YPet-NES	Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
138208	pcDNA3-PKCsub(T/A)-YPet-NES	Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
138210	pcDNA3-RLuc8C-FHA1-NES	Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.
138211	pcDNA3-mCherry-PKAsub-RLuc8N-NES	Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
138212	pcDNA3-PKAsub(T/A)-RLuc8N-NES	Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
138213	pcDNA3-mCherry-PKCsub-RLuc8N-NES	Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
138299	pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES	Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Antibodies from Article