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MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology.
Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S
Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123464pMK-PaccD (-82 to +23)MoChlo kit
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123465pMK-PaccD (functional)MoChlo kit
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123466pMK-PatpB (NEP functional PEP disabled)MoChlo kit
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123467pMK-PatpB (NEP)MoChlo kit
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123468pMK-PatpB (PEP and NEP functional)MoChlo kit
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123469pMK-PatpB (PEP functional NEP disabled)MoChlo kit
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123470pMK-PatpB (PEP)MoChlo kit
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123471pMK-PatpHMoChlo kit
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123472pMK-PatpI (NEP)MoChlo kit
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123473pMK-PatpI (PEP Hypothetical)MoChlo kit
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123474pMK-PclpPMoChlo kit
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123475pMK-PclpP (PEP)MoChlo kit
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123476pMK-IEEMoChlo kit
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123477pMK-Prrn (-64 to +17)MoChlo kit
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123478pMK-mPrrn (5-fold decrease transcription)MoChlo kit
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123479pMK-PpsbAMoChlo kit
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123480pMK-PpsbA (-42 to +9)MoChlo kit
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123481pMK-PpsbBMoChlo kit
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123482pMK-PpsbD-leaderMoChlo kit
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123483pMK-PpsbD-PGTbxMoChlo kit
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123484pMK-PpsbDMoChlo kit
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123485pMK-PrbcLMoChlo kit
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123486pMK-PrbcL (core -35 to +9)MoChlo kit
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123487pMK-PrbcL (-110 to +9)MoChlo kit
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123488pMK-PrpoBMoChlo kit
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123489pMK-Prrn16MoChlo kit
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123490pMK-Prrn (N. tabacum)MoChlo kit
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123491pMK-Prrn (N. tabacum) APrrn1MoChlo kit
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123492pMK-Prrn (N. tabacum) APrrn2MoChlo kit
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123493pMK-Prrn (N. tabacum) APrrn3MoChlo kit
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123494pMK-Prrn (N. tabacum) APrrn4MoChlo kit
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123495pMK-Prrn (N. tabacum) APrrn5MoChlo kit
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123496pMK-Prrn (N. tabacum) APrrn6MoChlo kit
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123497pMK-Prrn (N. tabacum) APrrn7MoChlo kit
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123498pMK-Prrn (N. tabacum) APrrn8MoChlo kit
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123499pMK-Prrn (N. tabacum) APrrn9MoChlo kit
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123500pMK-Prrn (N. tabacum) APrrn10MoChlo kit
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123501pMK-Prrn (N. tabacum) BPrrn12MoChlo kit
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123502pMK-Prrn (N. tabacum) BPrrn13MoChlo kit
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123503pMK-Prrn (N. tabacum) BPrrn14MoChlo kit
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123504pMK-Prrn (N. tabacum) BPrrn15MoChlo kit
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123505pMK-Prrn (N. tabacum) BPrrn16MoChlo kit
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123506pMK-Prrn (N. tabacum) BPrrn17MoChlo kit
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123507pMK-Prrn (N. tabacum) BPrrn18MoChlo kit
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123508pMK-Prrn (N. tabacum) BPrrn19MoChlo kit
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123509pMK-Prrn (N. tabacum) BPrrn20MoChlo kit
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123510pMK-Prrn (N. tabacum) BPrrn21MoChlo kit
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123511pMK-Zm PclpP::5'UTRMoChlo kit
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123512pMK-Zm PclpP::Gg10 leaderMoChlo kit
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123513pMK-PaccD::5'UTRMoChlo kit
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123514pMK-mPrrn::RBS (3.9%)MoChlo kit
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123515pOK-mPrrn::RBS (20%)MoChlo kit
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123516pMK-mPrrn::RBS (46%)MoChlo kit
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123517pMK-mPrrn::RBS (60%)MoChlo kit
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123518pMK-mPrrn::RBS (61%)MoChlo kit
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123519pMK-Prrn::RBSMoChlo kit
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123520pMK-atpB 5'UTRMoChlo kit
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123521pMK-atpB 5'UTR +10 (functional)MoChlo kit
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123522pMK-atpB 5'UTR +10 processed -90 (functional)MoChlo kit
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123523pMK-atpB 5'UTR TCR processed -90 (functional)MoChlo kit
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123524pMK-atpH 5'UTRMoChlo kit
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123525pMK-atpH 5'UTR processed -45MoChlo kit
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123526pMK-atpI 5'UTRMoChlo kit
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123527pMK-psbN 5'UTRMoChlo kit
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123528pMK-psbN 5'UTR processed -39MoChlo kit
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123529pMK-psbB 5'UTRMoChlo kit
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123530pMK-psbB 5'UTR processed -54MoChlo kit
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123531pMK-ndhD 5'UTRMoChlo kit
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123532pMK-rps2 5'UTRMoChlo kit
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123533pMK-rps2 5'UTR mAAGAMoChlo kit
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123534pMK-rps2 5'UTR mCCUCMoChlo kit
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123535pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)MoChlo kit
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123536pMK-rps2 5'UTR mCCUC (-20 to -10 deletion)MoChlo kit
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123537pMK-psbH 5'UTR + start codon + 1AAMoChlo kit
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123538pMK-psbA 5'UTR (SNM)MoChlo kit
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123539pMK-psbA 5'UTR (SEM)MoChlo kit
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123540pMK-psbA 5'UTR (delta SL)MoChlo kit
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123541pMK-psbA 5'UTR (delta 5'SL)MoChlo kit
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123542pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)MoChlo kit
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123543pMK-psbA-rbcL chimeric 5'UTR (R'SLWT)MoChlo kit
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123544pMK-rps14 5'UTRMoChlo kit
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123545pMK-rbcL 5'UTR (full)MoChlo kit
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123546pMK-rbcL 5'UTR (processed)MoChlo kit
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123547pMK-rbcL 5'UTR + 14 AAMoChlo kit
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123548pMK-T7G10 leaderMoChlo kit
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123549pMK-rrn16 5'UTR (native)MoChlo kit
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123550pMK-rrn16 5'UTR (+37)MoChlo kit
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123551pMK-psbA 5'UTRMoChlo kit
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123552pMK-Synthetic RBSMoChlo kit
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123553pMK-psbC 5'UTRMoChlo kit
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123554pMK-accD 5'UTRMoChlo kit
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123555pMK-clpP 5'UTRMoChlo kit
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123556pMK-mEmeraldMoChlo kit
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123557pMK-pporRFPMoChlo kit
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123558pMK-aadAMoChlo kit
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123559pMK-aphA6MoChlo kit
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123560pMK-nptIIMoChlo kit
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123561pMK-Myc aadA-GFPMoChlo kit
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123562pMK-FLARE16-S aadA-GFPMoChlo kit
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123563pMK-Lux-C codon opt. Nt chloroplastsMoChlo kit
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123564pMK-Lux-D codon opt. Nt chloroplastsMoChlo kit
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123565pMK-Lux-A codon opt. Nt chloroplastsMoChlo kit
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123566pMK-Lux-B codon opt. Nt chloroplastsMoChlo kit
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123567pMK-Lux-E codon opt. Nt chloroplastsMoChlo kit
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123568pMK-Lux-G codon opt. Nt chloroplastsMoChlo kit
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123569pMK-psbA 3'UTRMoChlo kit
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123570pMK-rbcL 3'UTRMoChlo kit
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123571pMK-petD 3'UTRMoChlo kit
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123572pMK-rpoA 3'UTRMoChlo kit
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123573pMK-E.coli rrnB 3'UTRMoChlo kit
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123574pMK-psaC 3'UTRMoChlo kit
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123575pMK-TMV 3'UTRMoChlo kit
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123576pMK-TYMV 3'UTRMoChlo kit
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123577pMK-BMV 3'UTRMoChlo kit
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123578pMK-ALMV 3'UTRMoChlo kit
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123579pMK-N. tabacum trnI/trnAMoChlo kit
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123580pMK-N. tabacum trnG/trnfMMoChlo kit
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123581pMK-N. tabacum trnV/3'rps12MoChlo kit
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123582pMK-N. tabacum atpB/accDMoChlo kit
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123583pMK-S. tuberosum trnI/trnAMoChlo kit
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123584pMK-S. tuberosum trnG/trnfMMoChlo kit
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123585pMK-S. tuberosum trnV/3'rps12MoChlo kit
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