Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.
Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S
Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. PubMed Article
Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S
Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123618 | pDDGFP-Leu2d-GgKDELR2 | Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123619 | pDDGFP-Leu2d-GgKDELR2 H12A | Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123620 | pDDGFP-Leu2d-GgKDELR2 R47A | Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123621 | pDDGFP-Leu2d-GgKDELR2 R47K | Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123622 | pDDGFP-Leu2d-GgKDELR2 E127A | Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123623 | pDDGFP-Leu2d-GgKDELR2 E127Q | Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123624 | pDDGFP-Leu2d-GgKDELR2 Y158F | Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123625 | pDDGFP-Leu2d-GgKDELR2 R159A | Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123626 | pDDGFP-Leu2d-GgKDELR2 R159K | Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection |
| 123627 | pBXPC3H-Syb37 | Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection |