Off-pathway-sensitive protein splicing screening based on a toxin/antitoxin system.
Beyer HM, Iwai H
Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139. PubMed Article
Beyer HM, Iwai H
Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124352 | pBHSTC97 | Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin |
| 124353 | pHBSTC159 | Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin |
| 124354 | pHYSTC299 | Plasmid expressing gp41-1 intein inserted into CcdA antitoxin |
| 124355 | pHBSTC147 | Plasmid expressing inactive gp41-1 intein inserted into CcdA antitoxin |
| 124356 | pBHSTC104 | Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin |
| 124357 | pHBSTC146 | Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin |
| 124358 | pBHDuet40 | IPTG-inducible vector encoding the N-terminal gp41-1 split fragment with N-terminally His-tagged GB1 |
| 124359 | pBHBAD42 | Arabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1 |