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Designing a green fluorogenic protease reporter by flipping a beta strand of GFP for imaging apoptosis in animals.
Zhang Q, Schepis A, Huang H, Yang J, Ma W, Torra J, Zhang SQ, Yang L, Wu H, Nonell S, Dong Z, Kornberg TB, Coughlin SR, Shu X
J Am Chem Soc. 2019 Mar 1. doi: 10.1021/jacs.8b13042.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
124428PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherryExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells
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124429PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherryExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells
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124430PCDNA3-GFP1-9 T2A mCherryExpresses GFP1-9 and T2A mCherry in mammalian cells
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124431PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherryExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish
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124432PCS2-FlipGFP(Casp3 cleavage seq)Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)
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124433pCS2-GFP1-9Expresses GFP1-9 in zebrafish
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124434pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherryExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila
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124436pcDNA3-FlipCherry-T2A-GFPExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells
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