Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks.
Rees HA, Yeh WH, Liu DR
Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4. PubMed Article
Rees HA, Yeh WH, Liu DR
Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125561 | hRad51(S208E-A209D)–Cas9(D10A) | Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125562 | hRad51(A190L-A192L)–Cas9(D10A) | Expresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125563 | hRad51(A89E)–Cas9(D10A) | Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125564 | hRad51(F86E)–Cas9(D10A) | Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125565 | hRad51(G151D)–Cas9(D10A) | Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125567 | Rad51–Cas9(D10A) | Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125568 | hRad51(K133R)–Cas9(D10A) | Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter |
| 125570 | CMV-hRad51 | Expresses the Rad51 protein in mammalian cells under CMV promoter |
| 125571 | hRad51(K133R) | Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter |