Rapid and Efficient CRISPR/Cas9-Based Mating-Type Switching of Saccharomyces cerevisiae.
Xie ZX, Mitchell LA, Liu HM, Li BZ, Liu D, Agmon N, Wu Y, Li X, Zhou X, Li B, Xiao WH, Ding MZ, Wang Y, Yuan YJ, Boeke JD
G3 (Bethesda). 2018 Jan 4;8(1):173-183. doi: 10.1534/g3.117.300347. PubMed Article
Xie ZX, Mitchell LA, Liu HM, Li BZ, Liu D, Agmon N, Wu Y, Li X, Zhou X, Li B, Xiao WH, Ding MZ, Wang Y, Yuan YJ, Boeke JD
G3 (Bethesda). 2018 Jan 4;8(1):173-183. doi: 10.1534/g3.117.300347. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169452 | pNA0306 | pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid |
| 169454 | pXZX351 | pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette |
| 169455 | pXZX501 | pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette |
| 169456 | pXZX352 | MATA fragment in pTOPO vector |
| 169457 | pXZX353 | MATα (alpha) fragment in pTOPO vector |