N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5.
Khatiwada B, Purslow JA, Underbakke ES, Venditti V
Protein Expr Purif. 2019 Nov 15;167:105540. doi: 10.1016/j.pep.2019.105540. PubMed Article
Khatiwada B, Purslow JA, Underbakke ES, Venditti V
Protein Expr Purif. 2019 Nov 15;167:105540. doi: 10.1016/j.pep.2019.105540. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125732 | pET-EIN-AlkBH5(66-292) | Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag. |
| 186466 | pET-EIN-FTO(32-505) | Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein) |