Plant gene editing through de novo induction of meristems.
Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 10.1038/s41587-019-0337-2. PubMed Article
Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 10.1038/s41587-019-0337-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127210 | pMM113 | BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS |
| 127211 | pMM114 | BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS |
| 127212 | pMM117 | BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS |
| 127213 | pMM119 | BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS |
| 127214 | pMM131 | BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS |
| 127215 | pMM134 | BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS |
| 127216 | pMM135 | BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS |
| 127217 | pMM136 | BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS |
| 127218 | pMM146 | BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS |
| 127219 | pMOD_C'5014 | Module C' with Pnos::WUS2 |
| 127220 | pMOD_D4910 | Module D with ZmUbi1::BBM |
| 127221 | pRN114 | BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM |
| 127222 | pRN227 | BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS |
| 127223 | pMOD_A5801 | 35S::Luciferase |
| 127224 | pMOD_A5803 | CmYLCV::Luciferase |
| 127225 | pMM100 | AtU6::sgRNA targeting PDS |
| 127226 | pMOD_C'0000a | Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit. |
| 127227 | pMM107 | 35S::IPT::pinII terminator |
| 127228 | pRN110 | CmYLCV::STM::35S terminator |
| 127229 | pMM112 | 35S::IPT::pinII terminator |
| 127230 | pMOD_D0000a | Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit. |
| 127231 | pRN104 | 35S::AFR5::35S terminator |
| 133312 | pMKV057 | BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT |
| 133313 | pMKV058 | BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2 |
| 133314 | pMKV059 | BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT |
| 133315 | pMKV060 | BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ |
| 160696 | pRN120 | BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator |