A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS).
Wu MR, Nissim L, Stupp D, Pery E, Binder-Nissim A, Weisinger K, Enghuus C, Palacios SR, Humphrey M, Zhang Z, Maria Novoa E, Kellis M, Weiss R, Rabkin SD, Tabach Y, Lu TK
Nat Commun. 2019 Jun 28;10(1):2880. doi: 10.1038/s41467-019-10912-8. PubMed Article
Wu MR, Nissim L, Stupp D, Pery E, Binder-Nissim A, Weisinger K, Enghuus C, Palacios SR, Humphrey M, Zhang Z, Maria Novoa E, Kellis M, Weiss R, Rabkin SD, Tabach Y, Lu TK
Nat Commun. 2019 Jun 28;10(1):2880. doi: 10.1038/s41467-019-10912-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127842 | SPECS library | For performing high-throughput cell state-specific synthetic promoter screening |
| 128569 | Accessory plasmid 1 | for mixing with SPECS library to produce lentivirus |
| 128571 | Accessory plasmid 2 | for cloning identified individual SPECS |