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Oskar Laur Lab: Emory Custom Cloning Core Plasmids - Oskar Laur
Unpublished

Plasmids from Article

ID Plasmid Purpose  
128023pCDNA3- c-terminal 6xMycMammalian expression vector with C terminal 6xMyc tag
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128024pcDNA3-c-terminal mouseFcMammalian expression vector with C terminal mFC tag
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128025pcDNA3-C-terminal GSTMammalian expression vector with C terminal GST tag
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128026pcDNA3-C-terminal CyPetMammalian expression vector with C terminal CyPet tag
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128027pcDNA3-C-terminal YPetMammalian expression vector with C terminal YPet tag
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128028pcDNA3-IL-2 signalMammalian expression vector with N terminal IL-2 signal peptide
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128029pcDNA3-IL-2 signal_CyPetMammalian expression vector with N terminal IL-2 signal peptide and CyPet
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128030pcDNA3-IL- signal_YPetMammalian expression vector with N terminal IL-2 signal peptide and YPet
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128031pcDNA3-IL-2 signal_rabbit FcMammalian expression vector with N terminal IL-2 signal peptide and rabbit Fc
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128032pcDNA3-IL-2 signal_GSTMammalian expression vector with N terminal IL-2 signal peptide and GST
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128033pcDNA3-IL-2signal-6xMycMammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag
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128034pcDNA3.1-HAMammalian expression vector with N terminal HA tag
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128035pVL1393-6xMycBaculovirus expression vector with C-terminal 6xMyc tag
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128036pShuttle-CMV-IRES-GFPAdenoviral shuttle vector with CMV promoter, coexpresses EGFP
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128037pBabe puro IRES-EGFPRetroviral vector with IRES-EGFP
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128038pBabe puro IRES-mCherryRetroviral vector with IRES-mCherry
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128040pcDNA3 DSred express2Mammalian expression vector with N terminal DsRed express2
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128041 pBABE puro EGFP Retroviral vector with N terminal EGFP
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128042pCDNA3 IL-2 signal mFcMammalian expression vector with C terminal IL-2 signal peptide and mFc
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128043pMA2780 lenti tet on EGFPTet inducible Lentiviral vector with EGFP co-expression
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128044pLEX-MCS PGK promoterLentiviral vector with PGK promoter
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128045pLEX-MCS UBC promoterLentiviral vector with UBC promoter
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128046pGL3basic luciferase and renilla_polyApGL3 basic luciferase reporter with co-expression of renilla luciferase
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128047pcDNA3.1 acGFPMammalian expression vector with N terminal AcGFP
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128048pLEX MCS snap tagLentiviral vector with snap tag
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128049pShuttle-CMV-GFP U6promoter Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
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128050pShuttle-CMV-GFP H1promoter Adenoviral shuttle vector for shRNA expression from H1 promoter with GFP marker
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128051pLex MCS mCherry MycLentiviral vector with mCherry Myc
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128052prs316 CUP-GFPYeast expression vector for N terminal GFP fusions
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128053pGL3 basic EGFPEGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basic
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128054pGL3 promoter EGFPEGFP reporter vector with restriction sites to clone in an enhancer of interest, derived from pGL3 promoter
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128055plenti guide ds redExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
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128057pcdna3.1 hygro mCherryMammalian expression vector with N terminal mCherry
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128058pFastbac1 Nterminal GSTBaculovirus expression vector with N terminal GST
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128059pCDH-EF1-IRES eGFPLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
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128060pScarlet-N1Mammalian expression vector for C terminal mScarlet-I fusions
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128061pLVX-Tet3G blasticidinLentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
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128062pLenti6-V5-Topo mCherryLentiviral vector for C terminal mCherry fusions
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128063plenti guide Thy1.1Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
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128064BS II KS+ loxP-GFP_ZEO_loxPhas loxp-GFP-Zeo-Loxp cassette
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128065pShuttle-CMV-GFPAdenoviral shuttle vector with CMV promoter-EGFP
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128067FUGW ds redLentiviral expression of dsRed Express2
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128068FUGW ECFPLentiviral expression of ECFP
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128069pREVtre GFPRetroviral tet inducible EGFP
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128070pLVX TRE 3G BFPLentiviral expression of Tet inducible TagBFP
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128071FUGW mplum3rd gen lentiviral plasmid with hUbC-driven mPlum
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128072pCDH-EF1-IRES plumgfp was exchanged to plum
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128073pLKO.1 mCherry puro exchanged to mCherry
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128074FUGW-EYFP 3rd gen lentiviral plasmid with hUbC-driven EYFP
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129427Lenti puro EF2 LUC-2A-Dendra2expression of LUC and Dendra2 reporters under EF2 promoter
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129428pLOC CMV-IRES-Dsred-blasticidinexpression of Dsred-blasticidin under CMVpromoter -IRES
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129429plex.mcs sv40promoter-lucLuc expression under SV40 promoter
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129431EF1a mCherryexpression of mCherry from EF1a promoter
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129432EF1a CHR2 mCherry WPREexpression of CHR2 mCherry from EF1a promoter
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129433pRS426 TEF mCherryexpress mcherry
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129435pTRE2 EGFPexpress GFP
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129436pCDH-UBC-LUC-EF1 Hygroexpress LUC
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129437pCDH-UBC-DSRED-LUC-EF1 Hygroexpress dsred-luc chimera
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129438pCDH-UBC-irfp luc-EF1 Hygroexpress irfp-luc chimera
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129439pCDH-UBC-dendra 2LUC-EF1 Hygroexpress dendra2-luc chimera
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129440pCDH-CMV-mCherry-EF1 Hygroexpress mCherry
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129441pGEX-4T1 NLS-GFPbacterial expression of GFP
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129442pTXB1 EGFPexpression of intein/ gfp
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129443EF1a- gfp-2a-puroexpression of GFP and Puro R
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129444pCDNA3.3 CAS9 IRES-Dsredexpression of FnCas9 and DsRed
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129445pET28a HAT epitopeexpression of HAT epitope
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