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amdS as a dominant recyclable marker in Cryptococcus neoformans.
Erpf PE, Stephenson CJ, Fraser JA
Fungal Genet Biol. 2019 Jun 17;131:103241. doi: 10.1016/j.fgb.2019.103241. PubMed Article

Plasmids from Article

ID Plasmid Purpose
128357pPEE7Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
128358pPEE8Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.
128359pPEE13Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.
128360pPEE16Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.
128361pPEE17Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
128362pPEE19Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the amdS2 counterselectable marker.
128363pPEE20Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the amdS2 counterselectable marker.
128364pPEE21Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the amdS2 counterselectable marker.
128365pPEE22Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the amdS2 counterselectable marker.
128366pPEE23Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the amdS2 counterselectable marker.
128367pPEE27Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128368pPEE28Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128369pPEE29Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128370pPEE30Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128371pPEE31Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128372pPEE32Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the NEO resistance marker
128373pPEE33Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the NEO resistance marker
128374pPEE34Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the NEO resistance marker
128375pPEE35Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the NEO resistance marker
128376pPEE36Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the NEO resistance marker
128377pPEE37Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the HYG resistance marker.
128378pPEE38Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the HYG resistance marker.
128379pPEE39Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the HYG resistance marker.
128380pPEE40Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the HYG resistance marker.
128381pPEE41Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the HYG resistance marker.

Antibodies from Article