On-demand biomanufacturing of protective conjugate vaccines.
Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP, Jewett MC
Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9444. doi: 10.1126/sciadv.abe9444. Print 2021 Feb. PubMed Article
Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP, Jewett MC
Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9444. doi: 10.1126/sciadv.abe9444. Print 2021 Feb. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128389 | pSF-CjPglB-LpxE | Expression of C. jejuni PglB and F. tularensis LpxE in E. coli |
| 128390 | pJL1-MBP-4xDQNAT | In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites |
| 128391 | pJL1-PD-4xDQNAT | In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites |
| 128392 | pJL1-PorA-4xDQNAT | In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites |
| 128393 | pJL1-TTc-4xDQNAT | In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites |
| 128394 | pJL1-TTlight-4xDQNAT | In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites |
| 128395 | pJL1-CRM197-4xDQNAT | In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites |
| 128397 | pJL1-EPA-DNNNS-DQNRT | In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites |
| 128398 | pTrc99S-ssDsbA-MBP-4xDQNAT | Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128399 | pTrc99S-ssDsbA-PD-4xDQNAT | Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128400 | pTrc99S-ssDsbA-PorA-4xDQNAT | Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128401 | pTrc99S-ssDsbA-TTc-4xDQNAT | Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128402 | pTrc99S-ssDsbA-TTlight-4xDQNAT | Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128403 | pTrc99S-ssDsbA-CRM197-4xDQNAT | Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli |
| 128404 | pTrc99S-ssDsbA-EPA-DNNNS-DQNRT | Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli |
| 132780 | CLM24 ∆lpxM | CLM24 strain with lpxM deletion |