Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J, Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L
Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. doi: 10.1016/j.cell.2020.11.040.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
128483pRSET-iSeroSnFRFluorescent reporter for serotonin (bacterial expression)
128484pDisplay-iSeroSnFRFluorescent reporter for serotonin (mammalian expression, membrane localization tag)
128485pAAV-CAG-iSeroSnFR-NlgnFluorescent reporter for serotonin (dendrite localization tag)
128486pAAV-CAG-FLEx-iSeroSnFRFluorescent reporter for serotonin (conditional + membrane localization tag)
129180pAAV-CAG-FLEx-iSeroSnFR-EnhancedExportFluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Recombinant Antibodies from Article