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Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors and Sources to Regulate Spontaneous Release.
Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
128812pFSW Doc2alpha WTTo make virus expressing Doc2alpha WT
128813pFSW Doc2alpha D291N mutantTo make virus expressing Doc2alpha D291 mutant
128814pFSW Doc2beta WTTo make virus expressing Doc2beta WT
128815pFSW Doc2beta D303N mutantTo make virus expressing Doc2beta D303N mutant
128816pFSW Doc2beta 6X mutantTo make virus expressing Doc2beta 6X mutant
128817pFSW Doc2alpha HalotagTo make virus expressing Doc2alpha Halotag
128818pFSW Doc2beta HalotagTo make virus expressing Doc2beta Halotag
128819pEF Doc2alpha WT GFPTo visualize Doc2alpha WT in PC12 cells
128820pEF Doc2beta WT GFPTo visualize Doc2beta WT in PC12 cells
128821pEF Doc2alpha D291N mutant GFPTo visualize Doc2alpha D291N mutant in PC12 cells
128822pEF Doc2beta D303N mutant GFPTo visualize Doc2beta D303N mutant in PC12 cells
128823pEF Doc2beta 6X mutant GFPTo visualize Doc2beta 6X mutant in PC12 cells
188980pEF Synaptophysin-mRubySynaptic buton marker
188981pEF Synaptophysin-GCamp6sPresynaptic fluorescent Ca2+ indicator

Antibodies from Article