Restoration of wild-type motility to flagellin-knockout Escherichia coli by varying promoter, copy number and induction strength in plasmid-based expression of flagellin.
Thomson NM, Pallen MJ
Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 10.1016/j.crbiot.2020.03.001. PubMed Article
Thomson NM, Pallen MJ
Curr Res Biotechnol. 2020 Nov;2:45-52. doi: 10.1016/j.crbiot.2020.03.001. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128848 | pJ211_WT_low | Low copy number plasmid for expression of E. coli flagellin from its natural promoter |
| 128849 | pJ211_WT_med | Medium copy number plasmid for expression of E. coli flagellin from its natural promoter |
| 128850 | pJ211_WT_hi | High copy number plasmid for expression of E. coli flagellin from its natural promoter |
| 128851 | pJ211_rha_WT_low | Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter |
| 128852 | pJ211_rha_WT_med | Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter |
| 128853 | pJ211_rha_WT_hi | High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter |
| 128855 | pJ291_WT_low | Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter |
| 128856 | pJ291_WT_med | Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter |
| 128857 | pD441_WT_hi | High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter |