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Controlling gene activation by enhancers through a drug-inducible topological insulator.
Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Hoshi K, Takato T, Toyoda A, Okano H, Hishikawa K
Elife. 2020 May 5;9. pii: 47980. doi: 10.7554/eLife.47980. PubMed Article

Plasmids from Article

ID Plasmid Purpose
129535pUC-STITCHSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.
129536pUC57-PB-PGK-tetR-KRAB-2A-PuroPiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.
129537pUC57-PB-PGK-tetR-3xFLAG-HA-2A-PuroPiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Antibodies from Article