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A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo.
Zhao N, Kamijo K, Fox PD, Oda H, Morisaki T, Sato Y, Kimura H, Stasevich TJ
Nat Commun. 2019 Jul 3;10(1):2947. doi: 10.1038/s41467-019-10846-1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
129590pCMV-15F11-HA-mEGFPThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
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129591pCMV-15F11-HA-mChThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
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129592pCMV-15F11-HA-HaloThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
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129593pET23b-15F11-HA-mEGFP-6xHisThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
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129594pET23b-2E2-HA-mEGFP-6xHisThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
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129595pCMV-2E2-HA-mEGFPThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
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129596pCMV-2E2-HA-mChThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
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Antibodies from Article