Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions.
Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PubMed Article
Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131171 | pOVC1 | Cloning vector containing A-B-C casette |
| 131172 | pOVC2 | Cloning vector containing A-C-B casette |
| 131173 | pOVC3 | Cloning vector containing B-A-C casette |
| 131174 | pOVC1_VfAU1-LOV_X | Expresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites |
| 131175 | pOVC1_CrPH-LOV_X | Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sites |
| 131176 | pOVC1_NcVVD-LOV_X | Expresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites |
| 131177 | pOVC1_RsLP-LOV_X | Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites |
| 131178 | pOVC1_MxCarH-CBD_X | Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites |
| 131179 | pOVC1_TtCarH-CBD_X | Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites |
| 131180 | pOVC1_AtCRY2-PHR_X | Expresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sites |
| 131181 | pOVC1_AtCIB_X | Expresses AtCIB (Arabidopsis thaliana) followed by restriction sites |
| 131182 | pOVC1_AtPIF6_X | Expresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites |
| 131183 | pOVC1_AtPHYB-S_X | Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites |
| 131184 | pOVC1_ScPH1-S_X | Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites |
| 131185 | pOVC1_X_VfAU1-LOV | Expresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites |
| 131186 | pOVC1_X_CrPH-LOV | Expresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites |
| 131187 | pOVC1_X_NcVVD-LOV | Expresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sites |
| 131188 | pOVC1_X_RsLP-LOV | Expresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites |
| 131189 | pOVC1_X_MxCarH-CBD | Expresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites |
| 131190 | pOVC1_X_TtCarH-CBD | Expresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites |
| 131191 | pOVC1_X_AtCRY2-PHR | Expresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sites |
| 131192 | pOVC1_X_AtPIF6 | Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites |
| 131193 | pOVC1_X_ScPH1-S | Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites |
| 131194 | pOVC1_X_AtCIB | Expresses AtCIB (Arabidopsis thaliana) preceded by restriction sites |
| 131208 | pOVC1_L1(GSSSG) | Cloning vector containing A-B-C casette with linker L1 in site B |
| 131209 | pOVC1_L2(GSSGGSSG) | Cloning vector containing A-B-C casette with linker L2 in site B |
| 131210 | pOVC1_L3(GSSSSGGSSSG) | Cloning vector containing A-B-C casette with linker L3 in site B |
| 131211 | pOVC1_L4(EAAAK)3 | Cloning vector containing A-B-C casette with linker L4 in site B |
| 131212 | pOVC1_EcSSPB-nano_X | Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites |
| 131213 | pOVC1_EcSSPB-micro_X | Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites |
| 131214 | pOVC1_HsPDZ1b_X | Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites |
| 131215 | pOVC1_X_AsPT1-LOV2-pep | Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites |
| 131216 | pOVC1_X_HsPDZ1b | Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites |
| 131217 | pOVC1_X_AsPT1-LOV2-EcSsra | Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites |
| 131218 | pOVC1_X_EcSSPB-micro | Expresses EcSSPB-nano (Escherichia coli) preceded by restriction sites |
| 131219 | pOVC1_X_EcSSPB-nano | Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites |
| 131220 | Opto-casp9_VfAU1-LOV_casp9 | Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9) |
| 131221 | Opto-casp9_AtCRY2-PHR-casp9 | Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9) |
| 131222 | Opto-casp9_casp9-VfAU1-LOV | Expresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9) |
| 131223 | Opto-casp9_casp9-AtCRY2-PHR | Opto-casp9_casp9-AtCRY2-PHR (light-activated caspase9) |
| 131224 | VfAU1-LOV_P2A_casp9 | Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site |