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Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions.
Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H
J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131171pOVC1Cloning vector containing A-B-C casette
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131172pOVC2Cloning vector containing A-C-B casette
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131173pOVC3Cloning vector containing B-A-C casette
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131174pOVC1_VfAU1-LOV_XExpresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites
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131175pOVC1_CrPH-LOV_XExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sites
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131176pOVC1_NcVVD-LOV_XExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sites
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131177pOVC1_RsLP-LOV_XExpresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites
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131178pOVC1_MxCarH-CBD_XExpresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites
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131179pOVC1_TtCarH-CBD_XExpresses TtCarH-CBD (Thermus termophilus) followed by restriction sites
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131180pOVC1_AtCRY2-PHR_XExpresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sites
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131181pOVC1_AtCIB_XExpresses AtCIB (Arabidopsis thaliana) followed by restriction sites
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131182pOVC1_AtPIF6_XExpresses AtPIF6 (Arabidopsis thaliana) followed by restriction sites
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131183pOVC1_AtPHYB-S_XExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites
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131184pOVC1_ScPH1-S_XExpresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites
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131185pOVC1_X_VfAU1-LOVExpresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites
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131186pOVC1_X_CrPH-LOVExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sites
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131187pOVC1_X_NcVVD-LOVExpresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sites
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131188pOVC1_X_RsLP-LOVExpresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sites
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131189pOVC1_X_MxCarH-CBDExpresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites
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131190pOVC1_X_TtCarH-CBDExpresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites
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131191pOVC1_X_AtCRY2-PHRExpresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sites
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131192pOVC1_X_AtPIF6Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites
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131193pOVC1_X_ScPH1-SExpresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites
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131194pOVC1_X_AtCIBExpresses AtCIB (Arabidopsis thaliana) preceded by restriction sites
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131208pOVC1_L1(GSSSG)Cloning vector containing A-B-C casette with linker L1 in site B
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131209pOVC1_L2(GSSGGSSG)Cloning vector containing A-B-C casette with linker L2 in site B
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131210pOVC1_L3(GSSSSGGSSSG)Cloning vector containing A-B-C casette with linker L3 in site B
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131211pOVC1_L4(EAAAK)3Cloning vector containing A-B-C casette with linker L4 in site B
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131212pOVC1_EcSSPB-nano_XExpresses EcSSPB-nano (Escherichia coli) followed by restriction sites
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131213pOVC1_EcSSPB-micro_XExpresses EcSSPB-micro (Escherichia coli) followed by restriction sites
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131214pOVC1_HsPDZ1b_XExpresses HsPDZ1b (Homo sapiens) folllwed by restriction sites
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131215pOVC1_X_AsPT1-LOV2-pepExpresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites
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131216pOVC1_X_HsPDZ1bExpresses HsPDZ1b (Homo sapiens) folllwed by restriction sites
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131217pOVC1_X_AsPT1-LOV2-EcSsraExpresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites
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131218pOVC1_X_EcSSPB-microExpresses EcSSPB-nano (Escherichia coli) preceded by restriction sites
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131219pOVC1_X_EcSSPB-nanoExpresses EcSSPB-micro (Escherichia coli) preceded by restriction sites
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131220Opto-casp9_VfAU1-LOV_casp9Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)
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131221Opto-casp9_AtCRY2-PHR-casp9Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)
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131222Opto-casp9_casp9-VfAU1-LOVExpresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)
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131223Opto-casp9_casp9-AtCRY2-PHROpto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)
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131224VfAU1-LOV_P2A_casp9Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site
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