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ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131471pORANGE Cloning template vectorCloning template form ORANGE method based knock-in constructs
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131472pORANGE C9orf4-GFP KIEndogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)
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131473pORANGE CACNG8-GFP KI #1Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)
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131474pORANGE CACNG8-GFP KI #2Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)
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131475pORANGE Cplx1-GFP KIEndogenous tagging of Complexin1: C-terminal (amino acid position: L128)
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131476pORANGE Cplx2-GFP KIEndogenous tagging of Complexin2: C-terminal (amino acid position: L128)
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131477pORANGE Dlg4-GFP KIEndogenous tagging of PSD95: C-terminal (amino acid position: R721)
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131478pORANGE Doc2A-GFP KIEndogenous tagging of Doc2a: C-terminal (amino acid position: L402)
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131479pORANGE GFP-Actb KIEndogenous tagging of βactin: N-terminal (amino acid position: D2)
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131480pORANGE GFP-CACNA1A KIEndogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)
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131481pORANGE GFP-CACNA1E KIEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)
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131482pORANGE GFP-CADPS KIEndogenous tagging of CAPS1: N-terminal (amino acid position: S39)
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131483pORANGE GFP-Clta KIEndogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)
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131484pORANGE GFP-Camk2a KIEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)
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131485pORANGE GFP-Grin1 KIEndogenous tagging of GluN1: N-terminal (amino acid position: A20)
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131486pORANGE GFP-Grin2a KIEndogenous tagging of GluN2a: N-terminal (amino acid position: A25)
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131487pORANGE GFP-Grin2b KIEndogenous tagging of GluN2b: N-terminal (amino acid position: S34)
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131488pORANGE GFP-Syt7 KIEndogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)
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131489pORANGE Gria1-GFP KIEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)
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131490pORANGE Gria2-GFP KIEndogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)
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131491pORANGE Gria3-GFP KIEndogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)
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131492pORANGE GSG1l-GFP KIEndogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)
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131493pORANGE mEos3.2-Camk2a KIEndogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)
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131494pORANGE Rims1-GFP KIEndogenous tagging of RIM1: C-terminal (amino acid position: S1615)
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131495pORANGE Rims2-GFP KIEndogenous tagging of RIM2: C-terminal (amino acid position: S1551)
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131496pORANGE Shank2-GFP KIEndogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)
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131497pORANGE Tubb3-GFP KIEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)
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131498pORANGE unc13a GFP KIEndogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)
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131499pORANGE GFP-RAB11a KIEndogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)
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131500pORANGE Shank1-GFP KIEndogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)
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131501pORANGE GFP-Nlgn3 KIEndogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)
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131502pORANGE WASF1-GFP KIEndogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)
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131503pORANGE GFP-Arpc5 KIEndogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)
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131504pORANGE CACNG2-GFP KIEndogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)
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131505pFUGW mCherry-KASHmCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in
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131506pFUGW SpCas9lentiviral expression of SpCas9 under hUBC promotor
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131507pFUGW ORANGE Gria1-GFP KI _ mCherry-KASHEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)
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131508pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASHEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)
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139651pORANGE LOX Cloning template vectorCloning template for ORANGE-CAKE conditional knock-in constructs
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139652pFSW FLEx mCherry-KASHConditional expression of mCherry-KASH
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139653pFSW FLEx Synapsin-FLAGConditional expression of Synapsin-FLAG
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139654pAAV MCS mCherry-KASHCloning template for AAV-based ORANGE knock-in constructs
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139655pAAV ORANGE Gria1-HaloTag KIEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)
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139656pAAV ORANGE Dlg4-HaloTag KIEndogenous tagging of PSD95: C-terminal (amino acid position: R721)
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139657pORANGE GFP-Pclo KIEndogenous tagging of Piccolo: N-terminal (amino acid position: G2)
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139658pORANGE GFP-Grin1 KI #2Endogenous tagging of GluN1: N-terminal (amino acid position: D23)
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139659pORANGE GFP-Grin1 KI #3Endogenous tagging of GluN1: N-terminal (amino acid position: A20)
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139660pORANGE GFP-Cacnb1 KIEndogenous tagging of CaVβ1: N-terminal (amino acid position: S8)
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139661pORANGE Cacnb2-GFP KIEndogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)
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139662pORANGE Cacnb3-GFP KIEndogenous tagging of CaVβ3: C-terminal (amino acid position: W479)
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139663pORANGE Cacnb4-GFP KIEndogenous tagging of CaVβ4: C-terminal (amino acid position: H417)
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139664pORANGE GFP-Bsn KIEndogenous tagging of Bassoon: N-terminal (amino acid position: L7)
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139665pORANGE Bsn-GFP KIEndogenous tagging of Bassoon: C-terminal (amino acid position: F3938)
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139666pORANGE GFP-Actb KI #2Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)
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