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Engineering a Model Cell for Rational Tuning of GPCR Signaling.
Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T
Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123024pWS060 Pheromone responsive promoter
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123025pWS053Pheromone responsive promoter
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123026pWS2247Tet promoter (TetO(x7)-pLEU2m)
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123027pWS1078Z3E promoter
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123028pWS480LexA UAS
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123029pWS481LexA UAS
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123030pWS482LexA UAS
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123031pWS483LexA UAS
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123032pWS484LexA UAS
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123033pWS485Core promoter
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123034pWS494Core promoter
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123035pWS491Core promoter
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123036pWS487Core promoter
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123037pWS493Core promoter
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123038pRC246Core promoter
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123039pWS486Core promoter
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123040pWS498Core promoter
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123041pWS496Core promoter
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123042pWS497Core promoter
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123043pWS495Core promoter
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123044pWS067_-factor GPCR
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123045pWS626P-factor GPCR
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123046pWS625Adenosine GPCR
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123047pWS1806Melatonin GPCR
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123048pWS2119PbPeptide GPCR
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123049pWS1037G_ subunit
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123050pWS936G_ subunit for creating C-terminal transplants
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123051pWS939Pheromone responsive transcription factor
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123052pWS926LexA bacterial repressor
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123053pWS931TetR bacterial repressor
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123054pWS930Beta-estradiol inducible DNA binding domain
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123055pWS932Pheromone responsive domain
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123056pWS033Green fluorescent protein
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123057pRC063Blue fluorescent protein
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123058pWS1837_-factor pheromone
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123059pWS788_-factor protease
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123060pWS081Regulator of G protein signalling
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123061pWS509Negative regulator of Fus3
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123062pWS510Negative regulator of Ste12
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123063pWS511Negative regulator of Ste12
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123064pWS627G___ bicistronic protein
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123065pWS1859Positive regulator of Ste20
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