Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9.
Choi GCG, Zhou P, Yuen CTL, Chan BKC, Xu F, Bao S, Chu HY, Thean D, Tan K, Wong KH, Zheng Z, Wong ASL
Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15. PubMed Article
Choi GCG, Zhou P, Yuen CTL, Chan BKC, Xu F, Bao S, Chu HY, Thean D, Tan K, Wong KH, Zheng Z, Wong ASL
Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131736 | pAWp63-clone32 (Opti-SpCas9) | Expresses Opti-SpCas9 in mammalian cells |
| 131737 | pAWp63-clone3-12 (OptiHF-SpCas9) | Expresses OptiHF-SpCas9 in mammalian cells |