Programmable RNA Targeting Using CasRx in Flies.
Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS
CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. PubMed Article
Buchman AB, Brogan DJ, Sun R, Yang T, Hsu PD, Akbari OS
CRISPR J. 2020 Jun;3(3):164-176. doi: 10.1089/crispr.2020.0018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132416 | OA-1050E (Ubiq-CasRx) | Express CasRx under Ubiquitin-63E promoter |
| 132417 | OA-1050R (Ubiq-dCasRx) | Express dCasRx under Ubiquitin-63E promoter |
| 132418 | OA-1050L (UASt-CasRx) | Express CasRx under UASt promoter |
| 132419 | OA-1050S (UASt-dCasRx) | Express dCasRx under UASt promoter |
| 132420 | OA-1050G (white) | express arrays of gRNA targeting White under dU6-3 promoter |
| 132421 | OA-1050I (notch) | express arrays of gRNA targeting Notch under dU6-3 promoter |
| 132422 | OA-1050K (firefly luciferase) | express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter |
| 132423 | OA-1050U (cinnabar) | express arrays of gRNA targeting Cinnabar under dU6-3 promoter |
| 132424 | OA-1050V (wingless) | express arrays of gRNA targeting Wingless under dU6-3 promoter |
| 132425 | OA-1050Z4 (yellow) | express arrays of gRNA targeting Yellow under dU6-3 promoter |
| 132426 | OA-1052B (Ubiq-Fluc_Ubiq-Rluc) | Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter |
| 133304 | OA-1050J (GFP) | express arrays of gRNA targeting GFP under dU6-3 promoter |