The MinDE system is a generic spatial cue for membrane protein distribution in vitro.
Ramm B, Glock P, Mucksch J, Blumhardt P, Garcia-Soriano DA, Heymann M, Schwille P
Nat Commun. 2018 Sep 26;9(1):3942. doi: 10.1038/s41467-018-06310-1. PubMed Article
Ramm B, Glock P, Mucksch J, Blumhardt P, Garcia-Soriano DA, Heymann M, Schwille P
Nat Commun. 2018 Sep 26;9(1):3942. doi: 10.1038/s41467-018-06310-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133624 | pET28a-mCherry-2xEcMTS | Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification. |
| 133625 | pET28a-mCherryGGBsMTS | Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification. |
| 133626 | pET28a-2xMreBN-mCherry-His | Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification. |