Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids.
Gaebler AM, Hennig A, Buczolich K, Weitz J, Welsch T, Stange DE, Pape K
J Vis Exp. 2020 Feb 18;(156). doi: 10.3791/60704. PubMed Article
Gaebler AM, Hennig A, Buczolich K, Weitz J, Welsch T, Stange DE, Pape K
J Vis Exp. 2020 Feb 18;(156). doi: 10.3791/60704. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134449 | px458_Conc2 | Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP |
| 134450 | px458_Conc3 | Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP |
| 134451 | px459V2.0_Conc2 | Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro |
| 134458 | px459V2.0_Conc3 | Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro |