Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin.
Tan W, Murphy VJ, Charron A, van Twest S, Sharp M, Constantinou A, Parker MW, Crismani W, Bythell-Douglas R, Deans AJ
PLoS One. 2020 Feb 24;15(2):e0229000. doi: 10.1371/journal.pone.0229000. eCollection 2020. PubMed Article
Tan W, Murphy VJ, Charron A, van Twest S, Sharp M, Constantinou A, Parker MW, Crismani W, Bythell-Douglas R, Deans AJ
PLoS One. 2020 Feb 24;15(2):e0229000. doi: 10.1371/journal.pone.0229000. eCollection 2020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134897 | pet16b-Avi-ubiquitin_rbs_BirA | Expresses HIS-Avi-tagged ubiquitin for recombinant protein production |
| 134898 | pET16b-PCNA | Bacterial expression and purification of human PCNA |
| 134904 | pFastbac1-Flag-FANCD2 | codon optimized expression and purification of human FANCD2 in insect cells using baculovirus |
| 137166 | pFastbac-Dual-StrepII-BARD1 | Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system |