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An optogenetic approach to control protein localization during embryogenesis of the sea urchin.
Uchida A, Yajima M
Dev Biol. 2018 Sep 1;441(1):19-30. doi: 10.1016/j.ydbio.2018.06.015. Epub 2018 Jun 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135597pSP64-mCherryExpression of mCherry mRNA for microinjection into S. purpuratus
135598pSP64-membrane-mCherryExpression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus
135599pSP64-membrane-mCherry-LOVExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus
135600pSP64-EMTB-GFP-ePDZExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus
135601pSP64-Lifeact-GFPExpression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus
135602pSP64-Lifeact-GFP-ePDZExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus
135603pSP64-GFP-ePDZ-CAAXExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus
135604pCS2-H2B-mCherryExpression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus
135605pSP64-H2B-GFPExpression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Antibodies from Article