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Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1.
Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y
FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135939pET28a His6Flag2AUF1(p37)Expresses AUF1p37 in E. coli
135940pET28a His6Flag2AUF1(p40)Expresses AUF1p40 in E. coli
135941pET28a His6Flag2AUF1(p42)Expresses AUF1p42 in E. coli
135942pET28a His6Flag2AUF1(p45)Expresses AUF1p45 in E. coli
135943pET28a His6HuRExpresses HuR in E. coli
135944pCS2+8 Flag2AUF1(p37)Expresses AUF1p37 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
135945pCS2+8 Flag2AUF1(p40)Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
135946pCS2+8 Flag2AUF1(p42)Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
135947pCS2+8 Flag2AUF1(p45)Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.
135950pCS2+8 Flag3HuRExpresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Antibodies from Article