Multi-functional genome-wide CRISPR system for high throughput genotype-phenotype mapping.
Lian J, Schultz C, Cao M, HamediRad M, Zhao H
Nat Commun. 2019 Dec 19;10(1):5794. doi: 10.1038/s41467-019-13621-4. PubMed Article
Lian J, Schultz C, Cao M, HamediRad M, Zhao H
Nat Commun. 2019 Dec 19;10(1):5794. doi: 10.1038/s41467-019-13621-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136989 | pAID6 | Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4 |
| 136992 | p426*-LbSgH | Helper plasmid for LbCpf1 gRNA cloning and expression |
| 136993 | p426*-SpSgH | Helper plasmid for SpCas9 gRNA cloning and expression |
| 136994 | p426*-SaSgH | Helper plasmid for SaCas9 gRNA cloning and expression |