Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.
Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA
Autophagy. 2020 Jan 23. doi: 10.1080/15548627.2020.1719746. PubMed Article
Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA
Autophagy. 2020 Jan 23. doi: 10.1080/15548627.2020.1719746. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137754 | GST-RFP-cb5 | Targets red flourescent protein (RFP) to the endoplasmic reticulum |
| 137755 | GST-Keima-cb5 | Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy |
| 137756 | HA-MAP1LC3A | Expression of LC3A |
| 137757 | HA-MAP1LC3B | Expression of LC3B |
| 137758 | HA-MAP1LC3C | Expression of LC3C |
| 137759 | HA-GABARAPL1 | Expression of GABARAPL1 |
| 137760 | HA-GABARAP | Expression of GABARAP |
| 137761 | HA-GABARAPL2 | Expression of GABARAPL2 |
| 137762 | NoTag MAP1LC3C | Expression of LC3C without epitope tags |
| 137763 | HA-GFP | Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency. |
| 137764 | GFP-DDK | Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency. |
| 137765 | GFP-CALCOCO1 | Expression and localization of tagged CALCOCO1 |
| 137766 | CALCOCO1-HA | Expression and localization of tagged CALCOCO1 |
| 137767 | CALCOCO1 L140A-mycDDK | CALCOCO1 CLIR mutant |
| 137768 | CALCOCO1 V142A-mycDDK | CALCOCO1 CLIR mutant |
| 137769 | CALCOCO1 W47A-mycDDK | CALCOCO1 LIR mutant |
| 137770 | CALCOCO1 R12H-mycDDK | CALCOCO1 R12H mutant |