Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.
Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D, Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L, Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q, Zhang Y, Qi Y
Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w. PubMed Article
Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D, Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L, Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q, Zhang Y, Qi Y
Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138094 | pYPQ131-STU-As | Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138095 | pYPQ131-STU-Fn | Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138096 | pYPQ131-STU-Lb | Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138097 | pYPQ132-STU-As | Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138098 | pYPQ132-STU-Fn | Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138099 | pYPQ132-STU-Lb | Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138100 | pYPQ133-STU-As | Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138101 | pYPQ133-STU-Fn | Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138102 | pYPQ133-STU-Lb | Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138103 | pYPQ134-STU-As | Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138104 | pYPQ134-STU-Fn | Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138105 | pYPQ134-STU-Lb | Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes |
| 138106 | pYPQ142-ZmUbi | Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter |
| 138107 | pYPQ143-ZmUbi | Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter |
| 138108 | pYPQ144-ZmUbi-pT | Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter |
| 138109 | pYPQ223-STU | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator |
| 138110 | pYPQ230-STU | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminator |
| 138111 | pYPQ233-STU | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator |
| 138112 | pYPQ239-STU | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminator |
| 138113 | pYPQ281 (MbCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter |
| 138114 | pYPQ282 (TsCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter |
| 138115 | pYPQ283 (MlCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter |
| 138116 | pYPQ284 (Mb2Cas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter |
| 138117 | pYPQ284-RVR | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter |
| 138118 | pYPQ284-v1 | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoter |
| 138119 | pYPQ284-v2 | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter |
| 138120 | pYPQ285 (Lb5Cas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter |
| 138121 | pYPQ285-RVR | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter |
| 138122 | pYPQ286 (CMaCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter |
| 138123 | pYPQ287 (BsCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter |
| 138124 | pYPQ287-RVR | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter |
| 138125 | pYPQ288 (BoCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter |
| 138126 | pYPQ289 (ErCas12a) | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter |
| 138127 | pYPQ289-RVR | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter |
| 160327 | pYPQ284-RVRR | Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter |