Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. PubMed Article
Watters KE, Shivram H, Fellmann C, Lew RJ, McMahon B, Doudna JA
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138281 | pKEW215_AcrIIA13 | Expression of AcrIIA13 protein |
| 138282 | pKEW353_AcrIIA13_truncated | Expression of truncated AcrIIA13 protein |
| 138283 | pKEW394_AcrIIA14 | Expression of AcrIIA14 protein |
| 138284 | pKEW395_AcrIIA14_truncated | Expression of truncated AcrIIA14 protein |
| 138285 | pKEW397_AcrIIA15 | Expression of AcrIIA15 protein |
| 138286 | pKEW398_AcrIIA15_truncated | Expression of truncated AcrIIA15 protein |
| 138305 | pCF525-AcrIIA4_gb39_lenti | Lenti expression of AcrIIA4 |
| 138306 | pCF525-AcrIIA5_gb36_lenti | Lenti expression of AcrIIA5 |
| 138310 | pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti | Lenti expression of AcrIIA13b |
| 138311 | pCF525-AcrIIA14_gb77_Cand9_lenti | Lenti expression of AcrIIA14 |
| 138312 | pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti | Lenti expression of AcrIIA14, C-terminus |
| 138313 | pCF525-AcrIIA15_gb79_Cand27_lenti | Lenti expression of AcrIIA15 |
| 138315 | pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti | Lenti expression of mTgBFP2 |
| 138316 | pCF820_U6-sgRNA-EF1a-mCherry2_lenti | Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP |
| 138317 | pCF823_EFS-Cas9-PuroR_lenti | Lenti expression of SpyCas9 with Puro selection |
| 138318 | pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti | Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP |