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Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.
Artegiani B, Hendriks D, Beumer J, Kok R, Zheng X, Joore I, Chuva de Sousa Lopes S, van Zon J, Tans S, Clevers H
Nat Cell Biol. 2020 Mar;22(3):321-331. doi: 10.1038/s41556-020-0472-5. Epub 2020 Mar 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
138567pCRISPR-HOT_tdTomatoUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ
138568pCRISPR-HOT-P2A-Clover-BlastRUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ
138569pCRISPR-HOT-Clover-BlastRUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Antibodies from Article