RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.
Yao S, Yuan P, Ouellette B, Zhou T, Mortrud M, Balaram P, Chatterjee S, Wang Y, Daigle TL, Tasic B, Kuang X, Gong H, Luo Q, Zeng S, Curtright A, Dhaka A, Kahan A, Gradinaru V, Chrapkiewicz R, Schnitzer M, Zeng H, Cetin A
Nat Methods. 2020 Mar 23. pii: 10.1038/s41592-020-0774-3. doi: 10.1038/s41592-020-0774-3. PubMed Article
Yao S, Yuan P, Ouellette B, Zhou T, Mortrud M, Balaram P, Chatterjee S, Wang Y, Daigle TL, Tasic B, Kuang X, Gong H, Luo Q, Zeng S, Curtright A, Dhaka A, Kahan A, Gradinaru V, Chrapkiewicz R, Schnitzer M, Zeng H, Cetin A
Nat Methods. 2020 Mar 23. pii: 10.1038/s41592-020-0774-3. doi: 10.1038/s41592-020-0774-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140131 | pAAV-EF1a-NCreV | can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer |
| 140132 | pAAV-EF1a-CCreV | can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer |
| 140133 | pAAV-EF1a-CDreV | can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer |
| 140135 | pAAV-EF1a-iCreV | can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase |
| 140136 | pAAV-EF1a-iDreV | can be used to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase |
| 140137 | pAAV-EF1a-iFlpV | can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase |